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- Lid geworden
- 26 feb 2004
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- Topic Starter
- #81
Goeie vraag Squibcake, of Myobolix.. , of wie je ook bent op dit forum..
je hebt toch al antwoord gekregen op Anabolic Review ?
hier heb je het antwoord nog eens:
Description
Long™R3IGF-I is a recombinant analog of human insulin-like
growth factor-I (IGF-I) that has been specifically engineered
for the enhancement of cell culture performance.
Long™R3IGF-I is more biologically potent in vitro than either
insulin or native IGF-I and has been documented to
significantly increase recombinant protein production. It is
ideal for both research and large-scale culture systems utilizing
serum-free or low-level serum applications. Long™R3IGF-I is
effective in any cell that contains type I IGF receptors, including
most Chinese Hamster Ovary (CHO) lines, hybridomas and
fibroblasts. Long™R3IGF-I is produced in a patented E. coli
expression system without the use of animal-derived
components.
Precautions
This product is for research/laboratory use. THIS PRODUCT
IS NOT INTENDED FOR HUMAN OR THERAPEUTIC USE.
Storage
Long™R3IGF-I in lyophilized form should be stored at
2 to 8 C and is stable for at least 3 years. Liquid solutions of
Long™R3IGF-I (prepared as described below) can be stored
at 2 to 8 C and are stable for at least 2 years.
Preparation Instructions
1. Long™R3IGF-I is supplied lyophilized in an atmosphere of
nitrogen at a slight vacuum (-25 kPa). Remove the metal
cap from the glass vial and introduce an air filled syringe
through the septum to equalize the pressure.
2. Add sufficient 10 mM HCl or 100 mM acetic acid solution
to the vial to achieve a peptide concentration of at least
0.1 mg/mL. Concentrations of 1 mg/mL or more are
recommended.
3. Mix the solution thoroughly to insure the peptide is
completely dissolved.
4. Filtration of a Long™R3IGF-I solution or a culture medium
after the addition of Long™R3IGF-I should be performed
using a low protein-binding membrane such as
Polyvinyl Idene Flouride (PVDF), Poly Ethylene Sulfite (PES)
or Cellulose Acetate (CA).
Methods for Use
Once Long™R3IGF-I has been reconstituted, it can then be
added directly to cell culture medium. A titration of
Long™R3IGF-I should be performed for each different
application as the optimum concentration may vary depending
upon the cell type used and other components present in the
medium. The recommended final concentration range of
Long™R3IGF-I is 10 to 50 µg/L (or ng/mL).
Because Long™R3IGF-I and insulin may compete for the same
cell receptors, the effectiveness of Long™R3IGF-I will be
masked if it is added in conjunction with commonly employed
concentrations of insulin (1 to 10 mg/L). However, inclusion
of physiological levels of insulin (5 to 10 µg/L) in cell culture
medium containing the recommended levels of
Long™R3IGF-I results in beneficial synergistic effects in certain
applications.
en de link naar jouw thread:
http://forums.steroid.com/showthread.php?t=185753
dus Muscle-Research heeft dan ook hetzelfde probleem?
je hebt toch al antwoord gekregen op Anabolic Review ?
hier heb je het antwoord nog eens:
Description
Long™R3IGF-I is a recombinant analog of human insulin-like
growth factor-I (IGF-I) that has been specifically engineered
for the enhancement of cell culture performance.
Long™R3IGF-I is more biologically potent in vitro than either
insulin or native IGF-I and has been documented to
significantly increase recombinant protein production. It is
ideal for both research and large-scale culture systems utilizing
serum-free or low-level serum applications. Long™R3IGF-I is
effective in any cell that contains type I IGF receptors, including
most Chinese Hamster Ovary (CHO) lines, hybridomas and
fibroblasts. Long™R3IGF-I is produced in a patented E. coli
expression system without the use of animal-derived
components.
Precautions
This product is for research/laboratory use. THIS PRODUCT
IS NOT INTENDED FOR HUMAN OR THERAPEUTIC USE.
Storage
Long™R3IGF-I in lyophilized form should be stored at
2 to 8 C and is stable for at least 3 years. Liquid solutions of
Long™R3IGF-I (prepared as described below) can be stored
at 2 to 8 C and are stable for at least 2 years.
Preparation Instructions
1. Long™R3IGF-I is supplied lyophilized in an atmosphere of
nitrogen at a slight vacuum (-25 kPa). Remove the metal
cap from the glass vial and introduce an air filled syringe
through the septum to equalize the pressure.
2. Add sufficient 10 mM HCl or 100 mM acetic acid solution
to the vial to achieve a peptide concentration of at least
0.1 mg/mL. Concentrations of 1 mg/mL or more are
recommended.
3. Mix the solution thoroughly to insure the peptide is
completely dissolved.
4. Filtration of a Long™R3IGF-I solution or a culture medium
after the addition of Long™R3IGF-I should be performed
using a low protein-binding membrane such as
Polyvinyl Idene Flouride (PVDF), Poly Ethylene Sulfite (PES)
or Cellulose Acetate (CA).
Methods for Use
Once Long™R3IGF-I has been reconstituted, it can then be
added directly to cell culture medium. A titration of
Long™R3IGF-I should be performed for each different
application as the optimum concentration may vary depending
upon the cell type used and other components present in the
medium. The recommended final concentration range of
Long™R3IGF-I is 10 to 50 µg/L (or ng/mL).
Because Long™R3IGF-I and insulin may compete for the same
cell receptors, the effectiveness of Long™R3IGF-I will be
masked if it is added in conjunction with commonly employed
concentrations of insulin (1 to 10 mg/L). However, inclusion
of physiological levels of insulin (5 to 10 µg/L) in cell culture
medium containing the recommended levels of
Long™R3IGF-I results in beneficial synergistic effects in certain
applications.
en de link naar jouw thread:
http://forums.steroid.com/showthread.php?t=185753
dus Muscle-Research heeft dan ook hetzelfde probleem?